Macrophage inflammatory protein (MIP)-1α is a cytokine which produces wide range of bioactivities such as proinflammatory, immunomodulatory, and hematopoietic modulatory actions. To determine whether MIP-1α acts as a negative regulator on the
functions of lymphocyte, [3H]-thymidine incorporation test and flow cytometric analysis were performed by using human tonsil T cell, human peripheral blood T cell, and murine cytolytic T lymphocyte (CTL) line CTLL-2,
@ES The results were as follow-
@EN 1. When human tonsil T lymphocytes were stimulated with anti-CD3 monoclonal antibody (mAb), rate of T cell proliferation was about four times increased. 200ng/ml of MIP-1α inhibited anti-CD3 mAb-mediated T cell growth as much as 60%
(P<0.05).
2. The suppression of human peripheral T cell proliferation produced by MIP-1α was dramatic, but variable among T cells derived from different individuals (40% ~90%).
3. MIP-1αinhibited the proliferation of murine CTL line CTLL-2 as much as 75% (P<0.001).
4. When the MIP-1α was added to human peripheral T cell, cell proportion of CD4+ helper T cell and CD+8 CTL were not noticeably affected. The expression level of CD4, not of Cd8, however, was down regulated by MIPα treatment (27% ~ 82%).
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